Friends and family will gather Wednesday evening to honor the memory of brother and sister Henry Carter, Jr., and Alexis Faye Carter, who died August 15 in an early morning apartment fire in Overland Park.A memorial visitation will be held from 5 to 8 p.m. Wednesday at Maple Hill Funeral Home in Kansas City, Kan. A mass will be held at 11 a.m. Thursday, August 24 at Guardian Angels Parish, 1310 Westport Road, Kansas City, Mo.An obituary posted by the children’s family is below:In loving memory of our Angels Henry Carter Jr. 5 years old and his sis Alexis Faye Carter 3 years old of Overland Parks Ks. passed away on August 15, 2017. They leave behind their beloved parents, mother Allyse Carter and Henry Carter Sr., their sister Aliyah Perez-Carter and their little brother LaRoy Carter. Their Grandparents Connie Perez-Mendoza and Ralph Mendoza, also Donna Faye Monroe and Henry Carter and great grandmother Guadalupe Casperson, their loveable Aunts and Uncles and numerous cousins. They are preceded in death by Uncle Ralph Nicholas Mendoza and grandparents William and Wanda Perez. Both Henry Jr. and Alexis love playing with balloons and flowers and love watching videos of the movie Trolls and their 2 dogs. A memorial visitation will be held on Wednesday, August 23, 2017 from 5-8:00pm at Maple Hill Funeral Home. There will also be mass services on Thursday August 24, 2017 at 11:00 am at Guardian Angels Parish, 1310 Westport Road, Kansas City, MO. The family was able to raise nearly $13,000 through a GoFundMe campaign that will go toward the memorial services.Overland Park Fire Department officials say the investigation into what caused the blaze is still under way.
Pinterest Learning and memory are crucial aspects of everyday life. When we learn, our neurons use chemical and molecular signals to change their shapes and strengthen connections between neurons, a process known as synaptic plasticity. In Ryohei Yasuda’s lab at Max Planck Florida Institute for Neuroscience (MPFI), scientists are working to understand how these molecules send messages throughout the neuron. To achieve this, his team is constantly working to develop high-resolution imaging techniques to visualize the activity and location of the molecules involved in the process.Ada Tang, Ph.D., a postdoctoral researcher in Yasuda’s lab, developed new molecular biosensors, which helped her visualize the activity of two signaling proteins crucial to synaptic plasticity, ERK and PKA. These proteins send messages to other proteins by adding a phosphate group to the target proteins. The team found that these proteins, which were already known to play a role in synaptic plasticity, learning, and memory, have surprising properties in their activity.The work was published in March 2017 in Neuron. Email Share on Twitter Share LinkedIn Share on Facebook Dendrites are thin extensions that come out of a neuron’s cell body and receive messages from other neurons. They branch out to form a tree-like structure, each branch typically extending tens of micrometers. They are covered by spines: tiny protrusions that receive inputs from other neurons and initiate molecular signals inside the cell. When a spine is strongly stimulated, it grows and strengthens to encode memories. Scientists have previously used traditional pharmacological methods such as western blotting to determine the activity of ERK and PKA averaged over many cells, but they haven’t been able to visualize the molecules directly in dendritic spines because of their small size.To design sensors sensitive enough to visualize these molecules, Tang created a new dye molecule, sREAChet, a modified dark but light-absorbing molecule. When she linked sREAChet with both green fluorescent protein (GFP) and a target peptide of the protein, she found that it could readout the activity of the protein with 2-3 times higher sensitivity compared to previous sensors. This made the sensitivity sufficient for imaging activity in single dendritic spines. “These sensors will be useful for researchers in a broad field of cell biology since ERK and PKA are involved in a variety of phenomena in cells and their abnormal activity is related to many diseases including cancer and mental diseases,” explained Yasuda.To demonstrate the usefulness of the new sensors, Yasuda’s team first stimulated individual dendritic spines, then used a special microscope called a 2-photon fluorescence lifetime microscope to visualize how ERK and PKA activity moves from a single spine. To their surprise, the team found the proteins’ activity did not stay within the individual spine, but spread much more than 10 micrometers, along the dendrite, influencing nearby spines. The spreading is estimated to be about several tens of micrometers and potentially extends throughout a branch of dendrites. The Yasuda Lab had previously shown that stimulating just a few spines could lead to ERK activation in the nucleus, but they didn’t know how this was achieved. This experiment showed that after these proteins are activated in a spine, the message spreads strongly over a long distance and potentially reaches the nucleus. “To find that PKA and ERK activation in spines is spreading for several tens of micrometers is certainly a surprising discovery for the field,” said Tang.The team has visualized an important step in the process, but there is still a long way to go to understanding the biochemical underpinnings of learning and memory.